Figure 5.

NF-κB activation up-regulates biglycan expression. A. Real-time PCR was carried out to detect the mRNA expression of biglycan. Data are expressed as mean ± S.E. from at least three experiments performed in triplicate. *P < 0.05 and **P < 0.01, vs SH-EP1 cells. B. SH-EP1 cells and I-κBαM cells were treated with 100 ng/ml IGF-1, and the culture medium was digested with chondroitinase ABCase. Total protein was then concentrated using StrataClean resin and subjected to western blot assay with antibody against biglycan.