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. 2015 Sep 15;7(9):1564–1573.

Figure 2.

Figure 2

miR-128 inhibits HCC cell proliferation. A. Real-time PCR analysis of expression of miR-128 in 7404 and 7721 cells. U6 was used as a loading control. B and C. miR-128 caused cell proliferatiod inhibition in 7404 and 7721 cells by MTT assay. D and E. miR-128 caused cell proliferation inhibition in BEL7404 and 7721 cells by colony formation assay. F and G. Quantitative analysis of G0/G1 proportion in 7404 and 7721 cells. Cell cycle was examined by flow cytometry analysis. H. G0/G1 phase cell cycle related protein expression including p21, p27 and CyclinD1 were determined by western blot in 7404 and 7721 cells. The experiments were repeated three times. Data are mean ± SD. **p < 0.01.