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. 2015 Sep 15;7(9):1564–1573.

Figure 4.

Figure 4

miR-128 downregulates ITGA2 and ITGA5 expression via directly targeting their 3’UTR. A. Sequence alignment of human ITGA2 and ITGA5 3’UTR with miR-128. B and C. miR-128 targeted the wild-type but not the mutant 3’UTR of ITGA2 and ITGA5. Luciferase activity assay of indicated 7404 and 7721 cells transfected with the pGL3-ITGA2-3’UTR or pGL3-ITGA5-3’UTR reporter with miR-128. D and E. Ectopic expression of miR-128 downregulated ITGA2 and ITGA5 mRNA expression in 7721 and 7404 cells as determined by real time RT-PCR. F. miR-128 decreased ITGA2 and ITGA5 protein level in 7721 and 7404 cells by western blot. Error bars represent from three independent experiments. **p < 0.01. *p < 0.05.