Double immunofluorescence staining results showing the colocalization of TFEB or Beclin-1 with β-tubulin III or GFAP. Representative confocal images were taken of gray matter (GM) of 108-day-old mice. WT (panel 1, 3, 5, and 7) and ALS (panel 2, 4, 6, and 8). Scale bar = 50 μm.