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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Transl Res. 2015 Apr 29;167(1):214–227. doi: 10.1016/j.trsl.2015.04.011

Figure 1. Assay Format.

Figure 1

Serum/plasma induced transcriptional bioassay. The optimized assay uses commercially supplied peripheral blood mononuclear cells (PBMC) of a single draw of a single well-characterized person representative of the normal population for human analyses and PBMC of Brown Norway rats for rat analyses. In a volume of 500ul, PBMC are cultured with patient serum or plasma (40% or 20% for human or rat analyses, respectively). Purified RNA (~100ng) from PBMC is labeled and hybridized to the appropriate Affymetrix GeneChip. The conditioned media are retained for other analyses. Signal intensities are normalized with Robust Multichip Analysis (RMA; www.bioconductor.org/) and complex analyses are conducted with other softwares.