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. Author manuscript; available in PMC: 2016 May 18.
Published in final edited form as: Mucosal Immunol. 2015 Apr 29;9(1):38–55. doi: 10.1038/mi.2015.34

Figure 6. CD11c-DTR-sensitive leukocytes are not required after antigen priming to maintain type 2-dependent granulomas and fibrosis in the lung.

Figure 6

(a) Wild-type C57Bl/6 mice were irradiated to ablate hematopoiesis and reconstituted with bone marrow cells from wild-type C57Bl/6, CD11b-DTR, CD11c-DTR or mice expressing both CD11b-DTR and CD11c-DTR transgenes (CD11b/c-DTR). Chimeric mice were primed and challenged with S. mansoni eggs, and half were treated with DTX (25ng/g) when indicated. All mice were harvested at D7. (b) Representative images of granulomas and collagen (blue) in lungs stained with Masson’s Trichrome (wild-type control n=8; wild-type DTX n=7; Cd11c-DTR control n=14; Cd11c-DTR DTX n=18; Cd11b-DTR control n=18; Cd11b-DTR DTX n=14; Cd11b/c-DTR control n=19; Cd11c-DTR DTX n=18). (c) Lung granuloma volume was scored by a pathologist blinded to groups and data presented as average granuloma volume/mouse. (d) Lung collagen was measured by hydroxyproline content of all primed and egg challenged groups. DTX treatment of CD11b-DTR mice reduced both secondary lung granuloma volumes and collagen content of the lung. In contrast, DTX treatment of CD11c-DTR or CD11b/c-DTR mice did not change granuloma volumes or collagen content of the lung compared to DTX treated wild-type or CD11b-DTR mice, respectively. Data are presented as median and statistical significance calculated using Mann-Whitney U test. * p<0.05, ** p<0.01, *** p<0.001. Results represent two independent experiments.