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. 2015 Oct 30;6:921. doi: 10.3389/fpls.2015.00921

FIGURE 2.

FIGURE 2

Analysis of methylation patterns and levels in Populus simonii under heat stress, cold stress, and in the control group. (A) The ratio of different MSAP band types: the dark red part represents MSAP bands with the same methylation pattern in heat, cold, and control groups; the orange part represents MSAP bands with different methylation patterns in heat, cold, and control groups. (B) Hierarchical clustering analysis of pattern of MSAP markers: Inline graphic represents unknown methylation pattern; Inline graphic represents hemi-methylated pattern; Inline graphic represents methylated pattern; Inline graphic represents unmethylated pattern. (C) The number of four MSAP patterns: Inline graphic represents unknown methylation pattern; Inline graphic represents hemi-methylated pattern; Inline graphic represents methylated pattern; Inline graphic represents unmethylated pattern. (D) Relative methylation level, relative hemi-methylated level, and relative total methylation level (see Materials and Methods for calculations). (E) Venn diagram showing the number of heat stress and cold stress loci with stress-specific DNA methylation.