Table 1.

Sequences of the PCR primers.

Gene	Sequences of primers
Foxp3	Forward 5′-CGTACACCCAGGAAAGACAG-3′
	Reverse 5′-ATCCAGGAGATGATCTGCTTG-3′
GITR	Forward 5′-GACGGTCACTGCAGACTTTG-3′
	Reverse 5′-GCCAT GACCAGGAAGATGAC-3′
CTLA4	Forward 5′-GGACGCAGATTTATGTCATTGATC- 3′
	Reverse 5′-CCAAGCTAACTGCGACAAGGA-3′
IFN-γ	Forward 5′-AGCTCTTCCTCATGGCTGTTTC-3′
	Reverse 5′-TGTTGCTGATGGCCTGATTGT-3′
IL-10	Forward 5′-ACTTGGGTTGCCAAGCCTTAT-3′
	Reverse 5′-ATCACTCTTCACCTGCTCCACT-3′
TGF-β	Forward 5′-GGATACCAACTATTGCTTCAGCTCC-3′
	Reverse 5′-AGGCTCCAAATATAGG GGCAGGGTC-3′
IRF-1	Forward 5′- CAGAGGAAAGAGAGAAAGTCC-3′
	Reverse 5′- CACACGGTGACAGTGCTGG-3′
IRF-4	Forward 5′- GTGACTGTGCCCTGGCTTAT-3'
	Reverse 5′- TGGACATGATCTGGGCAACC-3'
IRF-8	Forward 5′-AACTGTGCTCTGGGCTCATC-3'
	Reverse5′- CCTCCGGGAAGTGTCCCTTA-3'
Foxp3 promoter	Forward 5'-GTGGTGAGGGGAAGAAATCA-3'
	Reverse 5'-GATGAGTGTGTGCGCTGATAA-3'
GAPDH	Forward 5′-GTTGTCTCCTGCGACTTCAACA-3′
	Reverse 5′-TCTCTTGCTCAGTGTCCTTGCT-3′