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. 2015 Jul 7;73(7):ftv045. doi: 10.1093/femspd/ftv045

Figure 4.

Figure 4.

Effects of PKC δ silencing on IL-6 or IL-8 levels in supernatants of A549 cell cultures during incubation with P. brasiliensis. A549 cells were transfected with PKC δ siRNA #1 or with Negative Control siRNA (C) for 24 h, and then incubated with P. brasiliensis yeasts overnight. After incubation with fungi, culture supernatants were collected for IL-6 and IL-8 levels determination. A549 cells were harvested and lysed with TX-100 lysis buffer. Twenty-five micrograms of protein were loaded per well of 10% SDS-PAGE gels. (A) Silencing of PKC δ was confirmed by western blot using anti-PKC δ. Relative PKC δ expression was determined by densitometric analysis of bands. Values represent the ratio of the intensity of PKC δ band to the corresponding intensity of β-actin band. Blots are representative of two independent experiments. (B) IL-6 and IL-8 levels in culture supernatants were determined by ELISA. Values represent the mean of triplicates ± standard deviation. *P < 0.01 compared to C. These results are representative of two independent experiments.