Table 1.
Primary, secondary and isotype control antibodies for flow cytometry.
| Target molecule | Conjugate | Clone | Isotype |
|---|---|---|---|
| Primary antibody | |||
| CD14a | FITC | TUK4 | IgG2a |
| MHC class IIb | None | H42A | IgG2a |
| CD11ba | None | CC126 | IgG2b |
| CD163a | None | 2A 10/11 | IgG1 |
| CD5d | FITC | CC17 | IgG1 |
| B-B4 (B-cell marker)b | None | BAQ155A | IgG1 |
| γδ T-cell receptord | FITC | 86D | IgG1 |
| CD80c | FITC | BB1 | IgM,κ |
| CD86c | None | IT2.2 | IgG2b,κ |
| Secondary/ isotype control antibody | |||
| Anti-mouse IgG1c | APC | X56 | IgG1,κ |
| Anti-mouse IgG2be | APC | Polyclonal | – |
| Anti-mouse IgG2af | APC | Polyclonal | – |
| Mouse isotype IgG2ac | FITC | G155-178 | Mouse IgG2a,κ |
| Rat anti-mouse IgMc | FITC | II41 | Rat IgG2a,κ |
aSupplied by ABD Serotec,Oxford,UK; bsupplied by VMRD Inc, Pullman, WA; csupplied by BD Pharmingen, San Diego, CA; dprimary antibodies were produced from the cell lines (86D was the kind gift of Dr Garry Barcham, University of Melbourne) and conjugated to FITC using a Fluroreporter FITC labelling kit (Molecular probes); esupplied by Columbia Biosciences, Columbia, MD; fsupplied by Caltag Lab, Burlingame, CA.