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HIV-1 p17/MA induces angiogenesis and lymphangiogenesis in vivo. (A) 5-μm sections were stained with anti-mouse LYVE1-Biotin and anti-mouse Streptavidin FITC (eBioscience). (B) Immunofluorescence double staining of Matrigel slides with anti-CD31 and anti-LYVE1 antibodies to detect blood (red) and lymphatic (green) cells and microvessels. (C) 3D presentation of Z stacks (1 μm each) acquired by imaging blood and lymphatic vessels of p17 and p17S75X matrigel slides.
