Figure 5.

Identification and validation of cold-inducible transcripts from early and late stages of silique development in B. juncea. (A) Transcripts of cluster 1 (early stage) and cluster 5 (late stage) were compared and candidates that were either specific or common to both the stages of silique development were identified. (B) Heat map showing relative abundance of selected cold-inducible transcripts belonging to the early, late and core subsets as determined by qPCR. (C) Scatter plot showing correlation between digital and qPCR expression profiles. Pearson correlation coefficient (R) was calculated between log2 fold change values of qPCR and digital expression. The key for sample labels should be interpreted as x DAP_y, where x denotes number of days; DAP represents days after pollination and y denotes either unstressed siliques –“C” or duration of cold stress in hours –“h”.