Table 1.

Quality filtering and statistics of raw reads obtained in transcriptome libraries of B. juncea siliques exposed to low temperature for different durations.

S. No.	Sample	No. of raw reads	No. of HQ reads	Percentage (%)	Mapped reads	Percentage mapping (%)
1	5 DAP_C	34,996,934	33,098,872	94.58	23,672,240	71.52
2	5 DAP_6 h	46,283,995	43,352,879	93.67	31,094,528	71.72
3	5 DAP_12 h	27,407,889	21,593,463	78.79	15,463,603	71.61
4	10 DAP_C	31,916,211	30,033,245	94.10	21,664,687	72.14
5	10 DAP_6 h	38,077,842	35,335,917	92.80	25,477,680	72.10
6	10 DAP_12 h	20,851,323	16,264,475	78.00	11,803,985	72.58
7	15 DAP_C	36,410,242	34,111,247	93.69	24,585,074	72.07
8	15 DAP_6 h	40,661,329	37,646,457	92.59	27,322,541	72.58
9	15 DAP_12 h	44,470,147	36,420,384	81.90	26,589,923	73.01
10	20 DAP_C	54,387,436	51,174,780	94.09	37,227,245	72.75
11	20 DAP_6 h	55,097,716	51,238,446	93.00	37,445,913	73.08
12	20 DAP_12 h	48,736,907	38,808,054	79.63	28,375,618	73.12
13	25 DAP_C	34,719,115	28,553,791	82.24	20,805,257	72.86
14	25 DAP_6 h	46,760,019	37,279,174	79.72	26,906,383	72.18
15	25 DAP_12 h	92,720,961	78,646,125	84.82	58,031,931	73.79
16	30 DAP_C	45,741,906	37,042,168	80.98	26,990,691	72.86
17	30 DAP_6 h	63,185,861	50,328,733	79.65	37,024,361	73.57
18	30 DAP_12 h	58,774,016	50,083,797	85.21	36,735,973	73.35

Raw reads were filtered to remove adapter sequences and low quality reads using NGS QC tool kit. The percentage of reads retained after QC filtering is depicted in column 5.