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. 2015 Oct 30;6:869. doi: 10.3389/fpls.2015.00869

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Copyright © 2015 Ruszkowski, Nocek, Forlani and Dauter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kinetic analysis of the inhibition of MtP5CR by HEPES. Enzyme activity was measured at varying substrate concentrations in the presence of various inhibitor levels. Invariable substrates were fixed as in the standard mixture. Three replications were carried out for each treatment. Lines intersecting at the x-axis in the Lineweaver-Burk plots accounted for non-competitive inhibition types in all cases. Non-linear regression analysis of data [Michaelis-Menten fit, Lineweaver-Burk (double-reciprocal) transform, linear regression over Michaelis-Menten data, and non-competitive inhibition] was computed using Prism 6 for Windows, version 6.03.