Figure 8.

Coenzyme (ball-and-stick model) binding by MtP5CR. (A) The MtP5CR/NAD⁺ complex. (B) The close-up view of the ribose 2′O-bound phosphate in NADP⁺. Both figures are oriented as in Figure 1C. Green mesh areas represent omit difference F_o–F_c electron density maps contoured at the 4 σ level. Water molecules that take part in binding interactions are depicted as small red balls. Chain B is represented as a yellow semitransparent surface. Note that Thr129 belongs to chain A and is only covered by a part of the C-terminal domain of chain B. The surface of interacting amino acids is semitransparent in (A) and clipped in (B) so it does not obscure the coenzymes. Black dashed lines represent bonding protein-coenzyme interactions. Intramolecular interaction between the N atom of nicotinamide and O from pyrophosphate group of NAD⁺ is depicted as a black solid line at the bottom of (A). Distances are given in Å.