Figure 3.

Determination of amount of biofilm produced by B. pumilus SF214. (A) Cells were grown at the indicated temperatures in rich (LB; light gray bars) or sporulation-inducing (DS; dark gray bars) media for 48 h. Cells were then removed, wells were stained and washed, and the OD (570 nm) was determined. (B) Biofilm formation at 25 °C in LB medium supplemented with proteinase K or DNase I, as previously indicated [14].