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. 2015 Oct 21;13(10):6472–6488. doi: 10.3390/md13106472

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Pigmentation (A) and sporulation efficiency (B) of wild type and mutants of strain SF214. (A) Cells of wild type (wt), unpigmented (M4), over-pigmented (M2) mutants, and spontaneous revertant of M2 (M2R) were grown for 36 h at 25 °C on LB plate. (B) At the indicated times a sample of each growing culture (SF214 and M2R strain in black and white squares, respectively) was collected and split into two aliquots that were diluted and plated on LB plates. One of the aliquots was heat-treated to kill all vegetative cells before plating. For each time point, the sporulation efficiency is the percentage of cells growing after the heat treatment, considering as 100% the number of cells growing without the heat treatment.