Table 2.
Effects of MeOH and ACN on the CI-ELISA.
| PAX In: | Solvent Concentration a | IC50 (ng/mL) b | Relative Response (%) c | N d |
|---|---|---|---|---|
| PBS | 0% | 1.36 ± 0.16 | 100% | 20 |
| Methanol | 10% | 1.87 ± 0.21 | 72% | 6 |
| 20% | 3.03 ± 0.28 | 44% | 6 | |
| 30% | 4.03 ± 0.52 | 33% | 6 | |
| Acetonitrile | 5% | 3.72 ± 0.51 | 36% | 6 |
| 10% | 7.2 ± 1.1 | 18% | 6 | |
| 20% | 17.3 ± 3.3 | 7% | 6 |
a Volume percentage of the solvent used to prepare the standards. Because the standards were mixed (1 + 1) with mAb in the assays, the concentration of solvent present during the competitive step was one half of the concentrations listed; b Average IC50 ± 1 SD for PAX; c Response of PAX in the indicated solution relative to PAX in PBS. See Experimental Section 3.5, Equation (1); d Number of ELISA plates used to determine the summary statistic.