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. 2015 Sep 18;1(7):364–373. doi: 10.1021/acscentsci.5b00211

Figure 4.

Figure 4

Binding of (A) croconate and (B) glucuronate to GRY53/7HC elicits distinct changes in the fluorescent signature of GRY53/7HC. The fluorescent titrations were fit to a one-site binding equation giving a KD of 35 ± 1 μM and 1.6 ± 0.3 mM for croconate and glucuronate, respectively. In both cases, the KD is within error of the KI, 42 ± 10 μM for croconate12 and 1.5 ± 0.3 mM for glucuronate. The similarity of the KI and KD values suggests that the fluorescent titration is reporting on actual binding events.