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. 2015 Oct 30;5:15774. doi: 10.1038/srep15774

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(a) SDS-PAGE analysis (using a 12% gel) of ~5 μg of each of the pure PD proteins or the indicated mixtures. Lane 1, 6 × His-Hal3^PD (expected MW: 29.1 kDa); lane 2, 6 × His-Vhs3^PD (expected MW: 34.5 kDa); lane 3, co-expressed 6 × His-Hal3^PD/Cab3^PD (expected Cab3 MW: 23.3 kDa); lane 4, co-expressed 6 × His-Vhs3^PD/Cab3^PD; lane 5, co-expressed 6 × His-Hal3^PD/6 × His-Vhs3^PD/Cab3^PD; MW, molecular weight markers with molecular weights indicated. (b) In vitro PPCDC activity of ~60 nM of either HsCoaC, Hal3^PD (H), Vhs3^PD (V), Hal3^PD/Cab3^PD (H + C), Vhs3^PD/Cab3^PD (V + C), and co-expressed Hal3^PD/Vhs3^PD/Cab3^PD (H + V + C). The data represent the average of three separate experiments, with the error bars showing the standard deviation.