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. 2015 Oct 30;5:15774. doi: 10.1038/srep15774

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Copyright © 2015, Macmillan Publishers Limited

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(a) SDS-PAGE analyses (8% gel) of DSS-treated 6 × His-Hal3^PD (expected MWs: 58.2 kDa (dimer), 87.3 kDa (trimer)) and 6 × His-Vhs3^PD (expected MWs: 69.2 kDa (dimer), 103 kDa (trimer)). (b–f) Size exclusion chromatography (SEC) analyses of the purified PD proteins and mixtures: (b) HsCoaC; expected MWs: 25.6 kDa (monomer), 76.8 kDa (trimer) (c) Hal3^PD; expected MWs: 29.1 kDa (monomer), 87.3 kDa (trimer), 153 kDa (hexamer), 349.2 kDa (dodecamer) (d) Vhs3^PD; expected MWs: 34.6 kDa (monomer), 103 kDa (trimer) (e) Hal3^PD/Cab3^PD; expected MW: 26.2 kDa (average monomeric MW), 78.6 kDa (2 × Hal3^PD/1 × Cab3 trimer); 157.2 kDa (4 × Hal3^PD/2 × Cab3 hexamer) (f) Hal3^PD/Vhs3^PD/Cab3^PD; expected MWs: 29 kDa (average monomeric MW), 87 kDa (1 × Hal3^PD/1 × Vhs3^PD/1 × Cab3 trimer).