Figure 5. Vhs3 has a different secondary structure composition and mode of flavin binding.

(a) CD spectra of Hal3^PD (H), Vhs3^PD (V), Hal3^PD/Cab3^PD (H + C), Vhs3^PD/Cab3^PD (V + C), and co-expressed Hal3^PD/Vhs3^PD/Cab3^PD (H + V + C) in the UV range, highlighting the differences and similarities in their secondary structures. (b) CD spectra of the same proteins and mixtures obtained in the visible range, showing the differences and similarities in the environments of their flavin cofactors. The vertical dotted line shows the absorbance maximum of all the proteins—with the exception of Vhs3^PD—at 375 nm. (c) Absorbance spectra obtained simultaneously with the CD spectra in panel b. The legend is identical to that shown in (b).