. 2015 Oct 30;5:15774. doi: 10.1038/srep15774

Table 1. T_m-values determined based on the heat-induced changes in the PD proteins’ secondary structures and the heat-induced release of their flavin cofactors.

Protein/Mixture	T_m based onsecondary structurechanges^a(CD at 222 nm)			T_m based onflavin release^b(CD at 320 nm)
Protein/Mixture	T_m¹	T_m²	T_m³	T_m
HsCoaC	65.6	75.5	–	70.2
Hal3^PD	45.0	60.1	67.6	41.2
Vhs3^PD	63.2	78.4	–	78.1
Hal3^PD/Cab3^PD	55.8	–	–	47.1
Vhs3^PD/Cab3^PD	56.8	73.5	–	nd^c
Hal3^PD/Vhs3^PD/Cab3^PD	57.7	68.5	–	53.8

^aDetermined from the maxima observed in the first derivative plots (Fig. 6b) of the heat-induced melt curves shown in Fig. 6a. The value in bold indicates the value that is most likely physiologically relevant (see text for details).

^bDetermined by fitting the data shown in the plots in Fig. 6c to the Gibbs-Helmholtz equation (see Experimental for details).

^cnd, not determined.

Table 1. Tm-values determined based on the heat-induced changes in the PD proteins’ secondary structures and the heat-induced release of their flavin cofactors.

Table 1. T_m-values determined based on the heat-induced changes in the PD proteins’ secondary structures and the heat-induced release of their flavin cofactors.