Skip to main content
. 2015 Oct 30;5:15807. doi: 10.1038/srep15807

Figure 2. Amiodarone induced autophagy and increased autophagic flux in the early phase of liver regeneration after PHx.

Figure 2

Wild-type mice were intraperitoneally injected with vehicle (Veh), amiodarone (AD), or chloroquine (CQ) at 0.5 h before PHx or the sham operation and then once per day until 48 h. Liver tissues were harvested at 0 h, 12 h, 24 h, and 48 h after surgery, and the tissue extracts were analyzed for LC3-II, p62, and β-actin protein by Western blotting (A,B). Autophagic flux was quantified by subtracting LC3-II levels in mice without additional CQ treatment from the LC3-II levels in mice with additional CQ treatment. The 0 h-PHx condition without CQ treatment was set to represent an autophagic flux of 100% (C,D). Relative expression protein levels of LC3-II were calculated as the optical densities of their blots normalized to the β-actin blots, and the densitometric values are shown. The values are shown as the mean ± SD in the bar graph and compared by Student’s t test. #P < 0.05 versus 0 h-treated PHx (n = 6).