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. 2015 Oct 30;5:15807. doi: 10.1038/srep15807

Figure 3. Amiodarone enhanced liver growth and hepatocyte proliferation in the liver regeneration after PHx.

Figure 3

Wild-type mice were intraperitoneally injected with vehicle (Veh), amiodarone (AD), or chloroquine (CQ) at 0.5 h before PHx or sham operation and then once per day until 168 h. Liver tissues were harvested at 0–168 h after surgery. Liver-to-body-weight ratios were calculated (A). Liver sections at 24 h after the sham operation, PHx with Veh, AD, or CQ were stained with H&E; original magnification, 400X (B). Representative immunohistochemical staining of Ki67 is shown (C). The percentage of Ki67-positive nuclei in hepatocyte was counted under low-power fields (200 ×) in 15 random sections from at least six different mice (D). Liver tissues were harvested at 0 h, 12 h, 24 h, or 48 h after surgery, and the tissue extracts were analyzed for PCNA, cyclin A, B, D1, E, p21, TGF-β1, and β-actin protein by Western blotting (E,F). The values are shown as the mean ± SD in the bar graph and compared by Student’s t test. #P < 0.05 versus vehicle-treated PHx. Scale bar, 25 μm in B, 50 μm in C (n = 6).