Figure 5. Amiodarone alleviated liver injury after PHx.

Wild-type mice were intraperitoneally injected with vehicle (Veh), amiodarone (AD), or chloroquine (CQ) at 0.5 h before PHx or sham operation and then once per day until 72 h. Plasma samples were collected at 0–72 h after PHx and analyzed for ALT levels (A). Wild-type mice were given control or Atg7-specific siRNA before PHx and plasma samples were analyzed for ALT levels at 24 h (B). Liver tissues were harvested at 0–48 h after surgery, and Caspase-8 cleavage and Caspase-3 cleavage was assessed by Western blot analysis (C,D). Samples were collected at 0–48 h after PHx and analyzed for IL-6 and IL-8 levels in plasma (E,F) and hepatocytes (G,H). The values are shown as the mean ± SD in the bar graph and compared by Student’s t test; #P < 0.05 versus vehicle-treated PHx (n = 6).