Figure 8. Perforin-2 is required for in vivo survival after orogastric S. typhimurium challenge.
(A) Aggregated survival curves of 70 C57BL/6 × 129 × 1/SJV mice challenged with 105
S. typhimurium. (B) Aggregated survival curves of 45,129X1/SVJ mice challenged with 105
S. typhimurium. (C–F) Organ load five days after 105
S. typhimurium infection in C57BL/6 × 129 × 1/SJV mice in (C) blood, (D) small intestine, (E) liver, and (F) spleen. 
= MPEG1 (Perforin-2) wild-type animals (+/+), 
= MPEG1 (Perforin-2) heterozygous animals (+/−), •= MPEG1 (Perforin-2) knockout animals (−/−). Log-rank (Mantel–Cox) test was performed for A and B with statistical significance p < 0.0001. One-way ANOVA with Tukey post-hoc multiple comparisons was performed in C-F. *p < 0.05 as indicated.
Figure 8—figure supplement 1. Weight loss curves after in vivo orogastric S. typhimurium challenge.
Representative weight loss curves of 15 mice in each group challenged with 105
S. typhimurium. 
= MPEG1 (Perforin-2) wild-type (+/+) animals, 
= MPEG1 (Perforin-2) heterozygous (+/−) animals, •= MPEG1 (Perforin-2) knockout (−/−) animals. One-way ANOVA with Tukey post-hoc multiple comparisons was performed with *p < 0.05 between Perforin-2 knockout:Perforin-2 wild-type mice *p < 0.05 between Perforin-2 knockout:Perforin-2 wild-type and Perforin-2 knockout:Perforin-2 heterozygous mice. *p < 0.05 between Perforin-2 knockout:Perforin-2 wild-type, Perforin-2 knockout:Perforin-2 heterozygous, and Perforin-2 heterozygous:Perforin-2 wild-type.
= MPEG1 (Perforin-2) wild-type (+/+) animals, = MPEG1 (Perforin-2) heterozygous (+/−) animals, •= MPEG1 (Perforin-2) knockout (−/−) animals. One-way ANOVA with Tukey post-hoc multiple comparisons was performed with *p < 0.05 between Perforin-2 knockout:Perforin-2 wild-type mice *p < 0.05 between Perforin-2 knockout:Perforin-2 wild-type and Perforin-2 knockout:Perforin-2 heterozygous mice. *p < 0.05 between Perforin-2 knockout:Perforin-2 wild-type, Perforin-2 knockout:Perforin-2 heterozygous, and Perforin-2 heterozygous:Perforin-2 wild-type.Figure 8—figure supplement 2. Equal Colonization in feces 12 hr following 105 S. typhimurium inoculation.
Feces was collected, homogenized, serially diluted, plated, and enumerated from 30 animals of each genotype 12 hr after S. typhimurium oral-gastric inoculation. No statistical differences were observed in fecal shedding between groups at this time point. Statistical analysis was performed with one-way ANOVA with Tukey post-hoc multiple comparisons.
Figure 8—figure supplement 3. Organ load 60 hr after 105 S. typhimurium oral-gastric infection.
(A–D) Organs from 10 mice were collected 60 hr after S. typhimurium infection, weighed, homogenized, serially diluted, plated, and enumerated. Samples were normalized in weight to one another. (A) Blood was collected from cardiac puncture, (B) Small Intestine, (C) Liver, and (D) Spleen. All samples analyzed by Kruskal–Wallis test with Dunn's post-hoc multiple comparisons test. *p < 0.05.