Figure 5. The parg1-4 mutant root is more sensitive to bleomycin than that of Col-0.

(A) Comparison of the primary root length of Col-0 and the parg1-4 mutant under normal condition and 10 μg ml⁻¹ bleomycin treatment. (B) Propidium iodide-stained root tips of Col-0 and parg1-4 seedlings grown on plates without (control) or with 10 μg ml⁻¹ bleomycin for 4 days. The completely reddish-stained cells indicate the dead cells. Scale bars = 75 μm. (C) Statistical analysis of the primary root lengths of Col-0 and the parg1-4 mutant grown on plates with different concentrations of bleomycin for 10 days. Significant differences (t-test) compared to Col-0 under the same conditions are indicated by asterisks: *P < 0.05; **P < 0.01. (D) Propidium iodide-stained root tips of Col-0 and parg1-4 seedlings grown on plates without (control) or with 20 μg ml⁻¹ bleomycin for 10 days. The arrows indicate the boundary of meristematic zone at the root tip before and after bleomycin treatment. Scale bars = 100 μm.