Figure 6. The phenotype of parg1-4 is caused by the over-accumulation of PAR in vivo.

(A) The phenotype of parg1-4 can be rescued by the PARP inhibitor 3-AB. The plants were grown on 1/2 MS plate (control), or plates with 25 μg ml⁻¹ bleomycin, and 25 μg ml⁻¹ bleomycin plus 0.75 mM 3AB, respectively for two weeks. (B) Fresh weight per 8 seedlings of the parg1 mutant grown on the plates described in (A). Data were presented as means of three replicates ± SE. Significant differences (t-test) compared to Col-0 under the same conditions are indicated by asterisks: **P < 0.01. (C) Detection of poly(ADP-ribosyl)ated proteins in Col-0 and parg1-4 seedlings grown on plates described in (A). RuBisCo large subunit was used to indicate the protein loading amount. This experiment was repeated twice with similar results.