FIG 3 .

DC-autophagy does not alter preclinical myeloid infiltration or viral growth in the cornea. atg5^fl/fl CD11-cre (n = 10) and atg5^fl/fl (n = 7) mice were infected bilaterally with 2 × 10⁶ PFU per eye of HSV-1 (strain 17). (A and B) Corneas were harvested at 3 dpi and analyzed using flow cytometry for all myeloid cells (CD11b⁺), neutrophils (CD11b⁺ Ly6G⁺ Ly6C^low), or macrophages (CD11b⁺ Ly6G⁻ Ly6C⁺ or CD11b⁺ Ly6G⁺ Ly6C^high) (A) and quantified as percentage of cells (left) and total number of cells per cornea (right) (B). Data represent 3 independent experiments. (C) Viral titers of ocular tear film after challenge with 2 × 10⁶ PFU of HSV-1 (strain 17) in one eye. Data represent 5 similar experiments. Statistical significance was determined by unpaired parametric t test (B) and analysis of variance (C).