GAD67 mislocalization and drug-induced seizure are polymorphic responses in Toxoplasma-infected brains. (A to C) Representative images of coronal sections from mock-infected (A), type II ME49-infected (B), and type III CEP-infected (C) mice 30 days postinfection. Scale bars, 500 µm. Expanded images show high-magnification views of hippocampus CA3 regions. Scale bars, 50 µm. (D) Quantification of percent GAD67 staining following binarizing of images. *, P < 0.001 (one-way ANOVA with Tukey’s post hoc test; n ≥ 3 mice for each group). (E) Averages (± SEM) of seizure scores of mock-infected, type II ME49-infected, and type III CEP-infected mice (30 days postinfection) recorded every minute for 15 min after injection of PTZ. Each group represents score data for a minimum of 6 mice. *, statistically significant difference between type II ME49-infected and mock-infected mice only (P < 0.05 [one-way ANOVA with Tukey’s post hoc test]). #, statistically significant difference between type II ME49 and both mock-infected and type III CEP-infected mice (P < 0.05 [one-way ANOVA with Tukey’s post hoc test]).