Skip to main content
. 2015 Oct 27;6(6):e01446-15. doi: 10.1128/mBio.01446-15

FIG 3 .

FIG 3 

TgATG8-depleted tachyzoites are deficient in intracellular growth. (A) Plaque assays were carried out by infecting HFF monolayers with TATi1-Ku80Δ or cKd-TgATG8 or complemented cKd-TgATG8 parasites for 7 days with or without ATc. (B) Measurements of lysis plaque areas show a significant defect in the cKd-TgATG8 lytic cycle when parasites are incubated with ATc compared to that in the parental and complemented cell lines. AU, arbitrary units. Values are the mean ± the standard error of the mean of four experiments. (C) Intracellular growth of TATi1-Ku80Δ, cKd-TgATG8, and complemented cKd-TgATG8 parasites preincubated for 48 h in ATc and allowed to invade new HFFs, still in the presence of ATc. The number of parasites per vacuole was determined 24 h after inoculation. Values are the mean ± the standard error of the mean of three independent experiments, where 200 vacuoles were counted for each condition.