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. 2015 Oct 30;5:15923. doi: 10.1038/srep15923

Figure 2. Alteration in Sf-miR-31 expression corresponds with radiation-induced caspase activation and apoptosis.

Figure 2

(a) DIC microscopy images of Sf9 cells 24h following irradiation with different doses show presence of apoptotic bodies in cultures irradiated at 2000Gy–3000Gy. Images are representative of three independent experiments. (b) Sf9 cells were irradiated at different doses and lysed for caspase-3 activation assay. Doses ≥1000Gy induce significant caspase-3 activation in a dose-dependent manner. Data are mean ± SD of three independent experiments. (*P < 0.001, **P < 0.02); (c) Real Time quantitative PCR analysis shows alterations in Sf-miR-31 expression 24 h after irradiation at different doses. Expression reduced significantly at 200Gy whereas it increased in a dose-dependent manner at 1000Gy-3000Gy. Data are mean ± SD of three independent experiments (*P < 0.005, **P < 0.002).