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. 2015 Oct 30;5:15923. doi: 10.1038/srep15923

Figure 3. Inhibition of miR-31 decreases caspase-dependent Sf9 apoptosis.

Figure 3

(a) Sf9 cells were transfected with AS-miR-31 and irradiated at different doses 4–6 h later. Significant reduction in apoptosis is evident after Sf-miR-31 inhibition using antisense RNA at doses 2000Gy-3000Gy (16h–20 h post irradiation). Transfection control (TC) and scrambled antisense control (ASC) were used to confirm the specific effects of antisense-miR-31. Arrows depict apoptotic bodies. Images are representative of three independent experiments. (b) Fluorescence-based morphological analysis of harvested cells shows inhibition of radiation-induced cell death by AS-miR-31. Data are mean ± SD of three independent experiments. (c) Flow cytometric analysis of harvested cells shows inhibition of radiation-induced cell death by AS-miR-31. Data are mean ± SD of three independent experiments. (*P < 0.05) (d) Caspase-3 activity was also reduced in lethally irradiated cells by pre-transfection with AS-miR-31. Data are mean ± SD of three independent experiments. (**P < 0.02).