FIGURE 1.
Nucleotide sequences of the wild-type and germ-line mAb13 VH, D-JH, Vκ and Jκ gene segments (A); and deduced amino acid sequences of the wild-type and recombinant mAb13 VHDJH and VκJκ gene segments (B). The top sequence in each cluster is that of the germ-line gene sequence to which the remaining sequences of the cluster are compared. 13G12 and 13K20 are the autologous germ-line sequences. Dashes indicate identities. Solid lines on top of each cluster depict CDR. Small letters denote untranslated sequences. The sequences or reverse complementary sequences encompassed by the H-Ov2, 13-FR2, GIII-FR3, κ-Ov2, and κIII-FR3 primers are underlined. The 13G12, mAb13 VH segment, and mAb13 Vκ segment gene sequences are available from EMBL/GenBank under accession numbers D-16832, D-16833, and D-16834, respectively. For the EMBL/GenBank accession number of the germ-line 13K20 gene sequence see legend to Figure 3.