Skip to main content
. Author manuscript; available in PMC: 2015 Oct 30.
Published in final edited form as: J Immunol. 1995 Jan 1;154(1):226–238.

Table II.

Identity of the sequences of the 10 autologous germ-line VκIII genes to those of the germ-line kv3g, kv3g″, and kv325 genes, and to the sequence of mAb13 Vκ gene segment

Autologous Germ-line Genesa Heterologous Germ-line Genes (% Identity)b
mAb13, Vκ Segment
kv3g kv3″ kv325
13K20, 13K23 100c 98.6 95.5 97.3
13K11, 13K12 98.6 100 95.0 95.9
13K07, 13K09 98.2 99.4 96.4 95.4
13K04 97.3 96.3 95.0 94.5
13K18 95.5 95.5 100 92.8
13K16, 13K17 93.2 92.8 96.4 90.5
a

The 10 autologous germ-line VκIII genes were isolated from the PMN DNA of the IDDM patient whose B cells were used for the generation of mAb 13 (Casali et al., J. Immunol. 144:3741, 1990; Ikematsu et al., J. Immunol. 152:1430, 1994).

b

The germ-line kv3g and kv3g″ genes were originally reported by Pech and Zachau (Nucleic Acids Res. 12:9292, 1984) as Vg and Vg″, respectively. The germ-line kv325 was originally reported by Kipps et al. (Proc. Natl. Acad. Sci. USA 84:2916, 1987).

c

Percent of identity was calculated for the sequence spanning residues 22 to 243 (Fig. 3). Such sequence may (219 nucleotides in genes kv3g, kv3g″, 13K20/13K23, 13K11/13K12, 13K07/13K09) or may not (222 nucleotides in genes kv325, 13K16/13K17, 13K18) include the AGC triplet deletion in the CDR1 (Fig. 3).