Figure 6. Activated SFKs initiate negative-regulatory ITIM pathways.
Immunoblots show phosphorylation of negative regulatory proteins Sirpα, SHIP1, and SHP-1 in unprimed and IFN-γ-primed CskAS BMDMs treated with 3-IB-PP1 or zymosandep. See Figure 6—figure supplement 1 for evidence that the negative-regulatory adaptor Dok-3 is also activated in response to 3-IB-PP1 treatment.
Figure 6—figure supplement 1. Dok-3 activation by 3-IB-PP1.
Co-immunoprecipitation and immunoblot experiments show that 3-IB-PP1 treatment of CskAS BMDMs induces Dok-3 association with SHIP1 and Dok-3 tyrosine phosphorylation.