FIG 7.

Antioxidants decrease antibiotic/chelation-induced ROS levels and rescue growth. E. coli CP9 was cultured in BHI medium in the presence or absence of an antibiotic (VAN at 250 μg/ml or CTX at 62.5 ng/ml), DFP (150 μg/ml), or both, and growth (A and C) and ROS levels (B and D) were measured in the presence or absence of antioxidants (5 mM glutathione [GSH] and 5 mM ascorbate). Data are the mean values and standard deviations from at least six separate replicates. P values were determined by Student's t test.