FIG 4.

Deposition of S-layer proteins in the envelope of lcp variants. B. anthracis Sterne (WT) and isogenic variants carrying a single lcp gene were fixed in 4% buffered formalin at 8 h following inoculation of cultures in fresh medium. The localization of Sap (A) and EA1 (B) was observed with specific rabbit antisera as well as secondary antibody conjugates and counterstained with B-vancomycin to reveal the septal peptidoglycan. As controls, sap and eag mutants were also stained with their respective antisera. DIC, fluorescence, and merged microscopy images are shown for each strain. Scale bars represent 10 μm.