TABLE 1.
B. anthracis strains used in this study
| Strain genotype | Designation | Descriptiona | Reference or source |
|---|---|---|---|
| WT | Sterne 34F2 | WT (pXO1+, pXO2−) | 20 |
| Variants | |||
| lcpB2::aphA3 ΔlcpB3B4C lcpD::aad9 | lcpB1+ strain | lcpB3 (BAS0746) (deletion, nt 798568–799746), lcpB4 (BAS3381) (deletion, nt 3356447–3357475), lcpC (BAS5115) (deletion, nt 4995461–4994610), lcpB2 (BAS0572)::aphA3 (insertion, nt 619533), lcpD (BAS5047)::aad9 (insertion, nt 4919831) | This work |
| ΔlcpB1B3B4C lcpD::aad9/plcpB2 | lcpB2++ strain | lcpB1 (BAS1830) (deletion, nt 1856993–1857982), lcpB3 (BAS0746) (deletion, nt 798568–799746), lcpB4 (BAS3381) (deletion, nt 3356447–3357475), lcpC (BAS5115) (deletion, nt 4995461–4994610), lcpD (BAS5047)::aad9 (insertion, nt 4919831); plcpB2 is pWWW412 with lcpB2 (BAS0572) | This work |
| ΔlcpB1 lcpB2::aphA3 ΔlcpB4C lcpD::aad9 | lcpB3+ strain | lcpB1 (BAS1830) (deletion, nt 1856993–1857982), lcpB4 (BAS3381) (deletion, nt 3356447–3357475), lcpC (BAS5115) (deletion, nt 4995461–4994610), lcpB2 (BAS0572)::aphA3 (insertion, nt 619533), lcpD (BAS5047)::aad9 (insertion, nt 4919831) | This work |
| ΔlcpB1 lcpB2::aphA3 ΔlcpB3C lcpD::aad9 | lcpB4+ strain | lcpB1 (BAS1830) (deletion, nt 1856993–1857982), lcpB3 (BAS0746) (deletion, nt 798568–799746), lcpC (BAS5115) (deletion, nt 4995461–4994610), lcpB2 (BAS0572)::aphA3 (insertion, nt 619533), lcpD (BAS5047)::aad9 (insertion, nt 4919831) | This work |
| ΔlcpB1 lcpB2::aphA3 ΔlcpB3B4 lcpD::aad9 | lcpC+ strain | lcpB1 (BAS1830) (deletion, nt 1856993–1857982), lcpB3 (BAS0746) (deletion, nt 798568–799746), lcpB4 (BAS3381) (deletion, nt 3356447–3357475), lcpB2 (BAS0572)::aphA3 (insertion, nt 619533), lcpD (BAS5047)::aad9 (insertion, nt 4919831) | This work |
| ΔlcpB1 lcpB2::aphA3 ΔlcpB3B4C | lcpD+ strain | lcpB1 (BAS1830) (deletion, nt 1856993–1857982), lcpB3 (BAS0746) (deletion, nt 798568–799746), lcpB4 (BAS3381) (deletion, nt 3356447–3357475), lcpC (BAS5115) (deletion, nt 4995461–4994610), lcpB2 (BAS0572)::aphA3 (insertion, nt 619533) | This work |
| Δsap | sap strain | sap (BAS0841) (deletion, nt 896758–899063) | 27 |
| Δeag | eag strain | eag (BAS0842) (deletion, nt 899843–902414) | 27 |
| bslO::aad9 | bslO strain | bslO (BAS1683)::aad9 (insertion, nt 1703151) | 3 |
a
The nucleotide numbers provided in this column correspond to nucleotide positions in the sequenced genome of the Sterne strain as described in the NCBI databank under accession no. NC_005945.