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Immunoblotting of capsule. (A) Various mutants derived from the Newman P18L strain (CYL11481) were grown in TSB-0G for 4 h for capsule isolation. (B) Complementation of the rbsR mutant (CYL12834) with pML4233 (pML100-rbsR) for restoration of the capsule phenotype. Capsules were isolated from cultures grown in TSB-0G in the presence of 2.5 μg/ml chloramphenicol for 2 h and then induced with 200 ng/ml of ATc for 2 h.
