FIG 5.

Transcription analyses. Total RNA (5 μg) from each sample used for Northern blotting was denatured in formamide, applied to TBE-agarose gels, and hybridized with the Dig-labeled specific probe indicated below each blot. (A) Expression of rbsR in CYL11481 cultures grown to different OD₆₀₀s, as indicated. (B) Map of TSS and predicted promoter of rbsR. The SigB binding consensus is shown below, with capital letters denoting highly conserved nucleotides and lowercase letters denoting poorly conserved nucleotides (w = A or T). (C) Effects of RbsR on rbsU, rpi, and prs transcription. Total RNAs were prepared from 4-h cultures (OD₆₀₀, ∼4.0) of S. aureus CYL11481 or the rbsR mutant (CYL12834) and hybridized with specific probes, as indicated. The expression of rbsU was also assessed by complementation of rbsR by using pML100-rbsR (pML4233) in the presence of ATc.