FIG 3.

FasX negatively regulates the abundance of PrtF1 and PrtF2 on the GAS cell surface. Western blot analyses of cell wall protein fractions from the indicated GAS strains. Western blot analyses were performed using anti-PrtF1 (A) and anti-PrtF2 (B) antibodies. The protein-bound membranes were stained after transfer but prior to Western analysis for use as loading controls. Images shown are representative of five different experiments. Note that the laddering pattern of reactivity observed for PrtF1 is similar to that gained from a previous, unrelated analysis of this protein (59).