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Glutathione is necessary for rapid cystine import. ΔfliY (KCI1142), ΔfliY ΔgshA (KCI1210), ΔydjN (KCI1254), and ΔydjN ΔgshA (KCI1516) strains grown in sulfate medium were suspended to an OD600 of 0.2 in the same medium. The cells were incubated for 5 min with chloramphenicol at 37°C, and then [14C]cystine cocktail was added. Mean rates normalized to gshA+ strains, with error bars (standard errors of the means) from three independent experiments.
