Figure 2.

Up-regulation of NRF2 downstream targets both in the liver and kidney of control and acute arsenic-exposed mice. Mice were treated with 0, 5, 10 and 20 mg/kg NaAsO₂ intra-gastrically for the 6, 12, 24, 48 and 72 h, and the extracted proteins of liver and kidney were subjected to SDS-PAGE. Expressions of HO-1, GSTO1/2 and GCLC in the liver (A, B) and kidney (D, E) of mice were assessed by western blotting. β-Actin was blotted as the loading control. Total RNA of liver and kidney from each 6 h experimental group was isolated and real-time PCR were conducted. The mRNA levels of Hmox1, Gsto1 and Gclc in liver and kidney were shown as (C) and (F). Results were expressed as mean ± SD (n = 4), and independent experiments were carried out three times. * denotes p < 0.05 compared with control mice.