Table 2. Summary of results from two additional CKO projects using the optimized liquid culture CoSBR protocol.

Gene	BAC clone ID	KanR wells w/loxP	Retrievals w/lox	Retrievals with error-free loxP
Cdh11	RP23–60C23	34/95 (36%)	6/21 (29%)	6/6 (100%)
	RP23–35E14	2/95 (2%)	n.d.	n.d.
S100A8	RP23–6E18	60/95 (63%)	6/17 (35%)	5/6 (83%)
	RP23–189H20/229K18	51/95 (54%)	n.d.	n.d.

One or more BAC clones were used per project along with a matching lagging-strand oligo. Experiments were done in 96-well plates. Retrieval minipreps were analyzed for presence of loxP and loxP-positive CKO vectors were subsequently sequenced. n.d., not done.