Figure 3.

Kinetics of galactose induction in med17-444, -504 and WT strains. Yeast strains were grown in raffinose-supplemented medium at 30°C, then galactose was added, cultures were transferred at the same time to 37°C and samples were collected for ChIP experiments at indicated time points upon galactose induction (T0, T20, T40 and T60 min). Quantitative ChIP experiments were performed using anti-HA antibody (12CA5) against Mediator subunit Med5-HA (A), TBP-HA (C) or anti-Rpb1 Pol II antibody (8WG16) (B). The mean values and standard deviation (indicated by error bars) of three independent experiments are shown. Immunoprecipitated fragments from ChIP experiments were amplified with primers corresponding to the GAL1 gene promoter (GAL1 P) or ORF (GAL1 O). A nontranscribed region on chromosome V was used as a negative control.