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. 2015 Aug 14;43(19):9291–9305. doi: 10.1093/nar/gkv818

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 1. — Fluorescence intensity changes at 370 nm observed for gp32 binding to 25-mer ssDNA constructs with 2-AP dimer probes located at different positions, and a schematic model for the binding. (A) Normalized fluorescence intensity changes of the dimer probes for ssDNA constructs at 1 μM concentration as a function of gp32 monomers added per 25-mer ssDNA lattice. ₂₅B_2,3 construct (black, closed circles); ₂₅B_10,11 construct (red, open circles) and ₂₅B_23,24 construct (green, open squares). The dashed vertical line in (B) represents the point at which 3.0 gp32 monomers have been added per 25-mer ssDNA lattice. (B) Raw fluorescence intensity changes for the titration curves of the same ssDNA constructs as in Figure 1A (same color-coding). (C) Schematic model of possible binding modes of trimeric gp32 monomer clusters to these 25mer ssDNA lattices.