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. 2015 Aug 14;43(19):9291–9305. doi: 10.1093/nar/gkv818

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Circular Dichroism spectral changes for 25-mer ssDNA constructs with 2-AP dimer probes at various positions within the ssDNA lattice. Panels A–C show CD spectra of the various ssDNA constructs upon addition of increasing concentrations of gene 32 protein, expressed as gp32 monomers added per ssDNA lattice present. (A) ₂₅B_2,3 construct; (B) ₂₅B_10,11 construct; and (C) ₂₅B_23,24 construct. In each panel the color code for the CD spectra are as follows: blue: ssDNA construct only; red: 1 gp32 monomers per 25-mer ssDNA lattice; green: 2 gp32 monomers per lattice; and purple: 3 gp32 monomers per lattice. (D) Relative Δϵ/AP change at 325 nm for each construct with increasing concentrations of gp32. The x-axis is expressed in units of gp32 monomers added per ssDNA lattice. ₂₅B_2,3 construct (grey, open circles); ₂₅B_10,11 (cyan, open squares) and ₂₅B_23,24 (orange, closed circles).