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. 2015 Aug 14;43(19):9291–9305. doi: 10.1093/nar/gkv818

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© The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Acrylamide quenching Stern–Volmer plots of 2-AP dimer-labeled 25-mer-ssDNA constructs with increasing concentrations of gp32 monomer added per 25-mer ssDNA lattice. (A) ₂₅B_2,3 construct; (B) ₂₅B_10,11; and (C) ₂₅B_23,24. The individual plots within each panel correspond to the various input concentrations of gp32 expressed as gp32 monomers added per ssDNA lattice, and are (for each spectrum): blue: 0; red: 1; green: 2; purple: 3; pink: 4; and brown: 5 gp32 monomers added per 25-mer lattice. F_o/F (y-axis) corresponds to the fluorescence intensity in the absence of quencher divided by the fluorescence at the given quencher concentration and [Q] (x-axis) is the molar concentration of acrylamide monomers. The initial concentrations of the ssDNA lattice constructs in each titration were 1 μM. (D) Relative changes in the slope of the Stern–Volmer plots for each construct with increasing concentrations of gp32. ₂₅B_2,3 construct (grey, open circles); ₂₅B_10,11 (cyan, open squares) and ₂₅B_23,24 (orange, closed circles). The x-axis is expressed in units of gp32 monomers added per ssDNA lattice present.